DNAMAN predicts the pattern of restriction fragments that are separated by using agarose gel electrophoresis. DNAMAN assumes that electrophoresis is under ideal conditions, and all the fragments migrate in a logarithmic function of the inverse of their sizes
Choose the Restriction | Restriction Analysis menu to start restriction analysis on the one or multiple DNA sequences. Check the Results: Draw restriction pattern option.
After selecting restriction enzymes and pressing the Next button, the analysis results are shown in a graphic window. Depending on the number of DNA sequences and the number of enzymes you have selected, the restriction pattern may be different.
1)One DNA sequence cut with more than two enzymes:
The pattern shows all restriction patterns of the DNA cut by each enzyme.
2)One DNA sequence cut with two enzymes
The pattern shows all restriction patterns of single digestion of the DNA with each enzyme and double digestion with the two enzymes.
3) Two DNA sequences cut with two enzymes
The pattern shows all restriction patterns of single digestion of the two DNA sequences with each enzyme and double digestion with the two enzymes.
4) More than two DNA sequences cut with one enzyme
The pattern shows all restriction patterns of each DNA digested with the enzyme.
The fragment size range in the pattern diagram is from 1 bp to 50 kb. To change the resolution of the pattern, you may set different sizes at the bottom of gel. The default size of the bottom size is 100 bp. The Bottom control allows you to modify this value.
You can get detailed information about the fragments on the gel. Place the cursor on a fragment and click the left mouse button to open a menu box, the size of the fragment and the cutting enzymes are indicated in a pop-up box.
You may change the size and shape of the gel. Place the cursor on the low-right corner of the gel. When the cursor switches to , press the left mouse button and drag it to change the size and shape of the gel.
You can also move the gel within the graphic window. Place the cursor on the up-left corner of the gel. When the cursor switches to , press the left mouse button and drag the gel.