Importing oligo sequences from a text file is a convenient tool to add a large number of oligo records to a database.
You may use a text file produced by the DNAMAN program with the function of exporting sequences (see the next section). You may also choose the File | New command to open a new file and manually enter the information of oligo records. For a new text document, you must follow the format rules: 1) Each record is separated by “//”, 2) Leave a space after the name of each field 3) Other information about the oligo sequence. For example:
//
NAME ExampleA
SOURCE Example 1 seq.
MEMO PCR primer; from 4 to 27; sense; 0.5 mg stored in box1
LENGTH 23
GC% 56.5
TM 57.
SEQ GACTGCCACTTCCTCGATGAAGG
//
The information of each field must be in single line. It is necessary to provide the name and the sequence in a record. The information related to other fields can be ignored. For example:
//
NAME ExampleA
SOURCE Example 1 seq.
SEQ GACTGCCACTTCCTCGATGAAGG
//
NAME ExampleB
SEQ CCACTTTCACAGGCACAGGAGCA
//
Save this file by choosing the File | Save as command. You may also use any other word processor to create an oligo record text file.
To import the file to database, choose the Primer | Oligo Database | Import from Text File command to open the File to ODB dialog box.
In the dialog box, select an oligo database to add records. Then click the File button to open the File Open dialog box where select the file containing the oligo records. The password will be asked if the database is protected. Click the OK button in the dialog box to process. DNAMAN displays a message box indicating the number of records imported from the text file.
Use this function to overview or print out the records of a database. You may also save this file and import it to another oligo database.
Choosing the Primer | Oligo Database | Export to Text File command opens a Database dialog box for record selection.
Select the database you want to export. You may export all records of the database by clicking the Select All button. All the selected records will be exported into a text window. You may save or print out the text document.
Example:
//
NAME ExampleA
SOURCE Example 1 seq.
MEMO PCR primer; from 4 to 27; sense; 0.5 mg stored in box1
LENGTH 23
GC% 56.5
TM 57.0
SEQ GACTGCCACTTCCTCGATGAAGG