• Two sequence alignment
  • When you load two or more DNA or protein sequences into sequence channels, the Sequence | Alignment | Two Sequence Alignment menu is activated. You may accurately compare two sequences with this function. Another advantage of using this function is that you may quickly align any region of the two DNA or protein sequences by defining analysis regions (see the section VI.2).

    A dialog box appears by selecting the Sequence | Alignment | Two Sequence Alignment command. You should specify the following parameters.

    Click the OK button to perform alignment and display the alignment results in a text window. DNAMAN includes the percentage of homology in the result window.

    The identity (%) of the two sequences is calculated using the following formula:

                    Number of identical bases or AAs between two sequences
    Identity (%) =  覧覧覧覧覧覧覧覧覧覧覧覧                         ×100
                    Length of aligned sequence - Length of all gaps
    

    DNAMAN shows also the gaps introduced in the alignment.

               Number of gaps of both sequences

    Gap (%) = 覧覧覧覧覧覧覧覧 × 100 Length of aligned sequence