According to the options you have checked in the Restriction analysis page, DNAMAN shows the analysis results in different windows.
Restriction analysis with single DNA sequence
DNAMAN shows the number of enzymes that has been used for screening and the number of cutting sites found in the sequence. The analysis results are displayed in three parts:
1. The cutting sites listed in alphabetical order of restriction enzymes. Enzyme recognition sites and cutting number and position are also shown.
2. The cutting sites listed in position order.
3. The names of non-cutting enzymes are listed.
If you have selected only one or two enzymes, the sizes of the restriction fragments of the single and/or double digestion will be displayed.
For example, PstI and SacI are selected in restriction analysis. DNAMAN adds the following information in the result table:
Fragments generated by digestion (bp):
For the linear DNA:
Cut by PstI:424, 610, 792, 975, 2297
Cut by SacI:320, 1783, 2995
Double digestion: 94, 198, 226, 610, 698, 975, 2297
For the circle DNA:
Cut by PstI:424, 610, 792, 3272
Cut by SacI:320, 4778
Double digestion:94, 198, 226, 610, 698, 3272
If you have checked the Show sites on sequence option, DNAMAN displays the sequence and cutting sites in the Summary window. The first letter of enzymes is located exactly on top of each cleavage base.
Restriction analysis with multiple DNA sequences
If you perform restriction analysis with multiple sequences, DNAMAN shows a table with all cutting enzymes on each sequence. For example, the result of cutting pUC18 and Insert with following enzymes:
Restriction analysis on 2 sequences
Methylation: dam-No dcm-No
BalI TGG/CCA
Insert 1446
DraI TTT/AAA
pUC18 1094 1786 1805
Insert 3232 3251 3943
HindIII A/AGCTT
pUC18 281
Insert 29
MstI TGC/GCA
pUC18 426 1449
Insert 262 1358 1456 3588
NaeI GCC/GGC
Insert 403 771 931 1285
NarI GG/CGCC
pUC18 446
Insert 414 435 549 1206
DNAMAN draws a restriction map with all restriction enzyme sites available on the sequence. The map will be circular if you have checked the Circular option in the Target DNA section. The restriction site positions will be labeled on the names of restriction sites if you have checked the With enzyme position option in the Results section. The resulting map provides a starting point for drawing a complete DNA map.
See the section X.5 for Restriction pattern.