1. Results of restriction analysis
  2. According to the options you have checked in the Restriction analysis page, DNAMAN shows the analysis results in different windows.

  3. Summary in text window
  4. Restriction analysis with single DNA sequence

    DNAMAN shows the number of enzymes that has been used for screening and the number of cutting sites found in the sequence. The analysis results are displayed in three parts:

    1. The cutting sites listed in alphabetical order of restriction enzymes. Enzyme recognition sites and cutting number and position are also shown.

    2. The cutting sites listed in position order.

    3. The names of non-cutting enzymes are listed.

    If you have selected only one or two enzymes, the sizes of the restriction fragments of the single and/or double digestion will be displayed.

    For example, PstI and SacI are selected in restriction analysis. DNAMAN adds the following information in the result table:

    
    Fragments generated by digestion (bp):
    
    For the linear DNA:
    Cut by PstI:424,   610,   792,   975,  2297
    Cut by SacI:320,  1783,  2995
    
    Double digestion: 94,   198,    226,   610,   698,   975,  2297
    For the circle DNA:
    Cut by PstI:424,   610,   792,   3272
    Cut by SacI:320,  4778
    Double digestion:94,    198,    226,   610,   698,  3272
    

    If you have checked the Show sites on sequence option, DNAMAN displays the sequence and cutting sites in the Summary window. The first letter of enzymes is located exactly on top of each cleavage base.

    Restriction analysis with multiple DNA sequences

    If you perform restriction analysis with multiple sequences, DNAMAN shows a table with all cutting enzymes on each sequence. For example, the result of cutting pUC18 and Insert with following enzymes:

    Restriction analysis on 2 sequences
    Methylation: dam-No  dcm-No
    
    BalI        TGG/CCA
    Insert      1446  
    
    DraI        TTT/AAA
    pUC18       1094  1786  1805  
    Insert      3232  3251  3943  
    
    HindIII     A/AGCTT
    pUC18       281   
    Insert      29    
    
    MstI        TGC/GCA
    pUC18       426   1449  
    Insert      262   1358  1456  3588  
    
    NaeI        GCC/GGC
    Insert      403   771   931   1285  
    
    NarI        GG/CGCC
    pUC18       446   
    Insert      414   435   549   1206
  5. Restriction map
  6. DNAMAN draws a restriction map with all restriction enzyme sites available on the sequence. The map will be circular if you have checked the Circular option in the Target DNA section. The restriction site positions will be labeled on the names of restriction sites if you have checked the With enzyme position option in the Results section. The resulting map provides a starting point for drawing a complete DNA map.

  7. Restriction pattern in graphic window
  8. See the section X.5 for Restriction pattern.