Some of data information used in DNAMAN, such as restriction enzyme databases and amino acid properties, can be accessed and modified by users. When you become an expert of DNAMAN, you can make some modifications to take advantage of the software.
- Restriction enzymes
DNAMAN allows users to create and edit custom enzyme data files (see the section X.2).
Displaying restriction enzyme information
- Choose Info | Restriction Enzymes to open the Restriction Enzymes dialog box.
- Select an enzyme file in the File box.
- In the Enzyme List box, clicking an enzyme name displays the related enzyme information:
- Recognizing sequence and cut position
- Available from which company
- Isoschizomers
- Compatible ends
- Miscellanies
Editing restriction enzyme information
- Choose the Info | Restriction Enzymes command to display Enzyme Information box.
- Select an enzyme file in the File box.
- Select the enzyme name you want to edit from Enzyme List box.
- Click the Edit button and the Edit Record dialog box appears.
- Edit the related information.
- There are the many suppliers’ names are listed in the Commercial source box:
- If you want to add a commercial source that is in the list, click that name.
- If you want to add a new commercial source that is not in the list, click the Add Source button. In the New Commercial Source box, type the name you want to provide.
- Click the Save button.
Adding new restriction enzyme
- Choose the Info | Restriction Enzymes command to display Enzyme Information box.
- Select an enzyme file in the File box.
- Click the Add button to open the Add Record dialog box.
- Fill in the boxes of the Name, Recognition sequence, Cut position, Isoschizomer, Compatible enzymes and Miscellanies.
- .Select the names of the available commercial source. Click the Add Source button to add a new name.
- Click the Save button.
Removing a restriction enzyme from the list
- Choose the Info | Restriction Enzymes command to display Enzyme Information box.
- Select an enzyme file in the File box.
- Select the enzyme name you want to remove from Enzyme List box.
- Click the Remove button to delete that enzyme from the list.
- Enzyme file conversion
You may convert an enzyme file from GCG format to DNAMAN format. This is especially useful when you like to import a REBase file of New England Biolabs to DNAMAN. You may download the REBase file from the Internet using GCG format and use this function to import to DNAMAN.
- Choose the Info | Convert Enzyme File command to display the GCG RE file to DNAMAN box.
- Press the File button to select a GCG restriction enzyme file in the File box.
- Enter a file name for DNAMAN restriction enzyme file. Give a name only. Do not enter any extension name. DNAMAN will automatically add a required extension name to it.
- Click the OK button.