Sequence editing
DNAMAN provides a text editor to edit sequence files. You may add or remove any text or sequence in files. You may also use Copy and Paste functions for editing the files. Some sequence analysis results are also shown as text file, therefore, these results can be edited using the editor. When you open a sequence file, the sequence may be loaded directly to the current channel. If the auto-load option is turned off, analysis functions are not immediately available. You must load manually the sequence into a channel prior to analysis.
Sequence analysis
You may choose a sequence once and perform many kinds of analyses on it. This sequence must be loaded into a sequence channel. DNAMAN provides 20 sequence channels to keep active sequences in memory. You can simultaneously analyze and compare all sequences in the channels. You may switch one sequence to another during analysis without going back to disk files.
Sequence alignment and comparison
You may directly use disk files for multiple alignment and sequence assembly. It is not necessary to load these sequences to channels. To perform these tasks, choose the corresponding commands on the menu bar, select your sequence files and fill out appropriate parameters. DNAMAN remembers the files used in these analyses. You may re-analyze these sequences again without going back to disk files.
If you use DNAMAN for the first time, you may follow the instructions described below.
To perform sequence analysis:
1) Launch DNAMAN. An example sequence file is automatically opened and you may go to Step 3. Otherwise, choose the Sequence | Load Sequence | From Sequence File menu.
2) Select the Example1.Seq file from Open File Dialog Box. Click Open to load Example1 to Sequence channel 1.
3) Choose the Restriction | Restriction Analysis menu to perform restriction analysis on Example1.
4) After restriction analysis, choose the Protein | Translation menu to translate Example1 to amino acid sequence.
To draw a restriction map without sequence:
Choose the Restriction | Draw Map menu. A plasmid map is drawn in a restriction map window. Double-click on the window to display the map properties and make modification.
Tips: All objects in a restriction map can be moved around by drag-and-drop. Restriction sites and Text objects can be aligned when they are selected.
The DNAMAN Help provides on-line access to the detailed information of DNAMAN functions and the instructions on how to use them. Choose the Help | Help Topics command to open the Help window. You can also select the Help | Index command, then go directly to related index section.